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[Chemical Knowledge]：Note on manual injection operation of gas chromatography

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Manual injection is a very common procedure when performing gas chromatography. The correct manual injection operation can ensure that the sample is accurately injected into the gas chromatograph and obtain accurate and reliable analysis results. However, manual injection is also an error-prone link, requiring the operator to strictly abide by some precautions. This article will focus on several key points that need to be paid attention to when manual injection of gas chromatography.

to ensure that the injection needle is clean. Before manually injecting, make sure that the injection needle is clean and there are no remnants of the last used sample. If necessary, the injection needle can be cleaned by washing with detergent and water, then rinsed with some common organic solvents, and finally dried with gas. This can effectively avoid the contamination of the new sample analysis caused by the residue of the previous sample.

pay attention to the depth and speed of injection. The depth and speed of injection have an important influence on the analysis results. If the sample is injected too fast or the injection needle does not reach the bottom of the sample, the analysis results will be inaccurate. Therefore, when performing manual injection, it is important to control the depth and speed of injection to ensure that the sample can be fully injected into the column to obtain accurate analysis results.

In addition, you also need to pay attention to the air pressure and temperature during injection. During the injection process, special attention should be paid to changes in air pressure and temperature. Changes in air pressure can affect the speed and depth of injection, while changes in temperature can affect the volatility of the sample. Therefore, before performing manual injection, it is necessary to stabilize the air pressure and temperature, which can be achieved by adjusting the air pressure and temperature parameters of the chromatograph. When the

performs manual injection, the operator should keep the pulse of the hand stable and move gently. Manual injection requires a steady, gentle movement of the operator's hand to avoid unnecessary damage to the column and injection needle. At the same time, after the injection, the injection needle should be cleaned and maintained in time to ensure that the accurate analysis results can be obtained in the next use. Manual injection of

gas chromatography is a critical operation step, which requires the operator to strictly abide by some precautions. The accuracy and stability of manual injection can only be ensured if the operating procedures are strictly followed. It is hoped that the precautions introduced in this article can help readers to better carry out manual injection operation of gas chromatography.

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