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[Chemical Knowledge]：The correct use of fluorescence spectrophotometer

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Fluorescence spectrophotometer is an instrument used to measure the fluorescence intensity of substances, which is widely used in the fields of biomedicine, environmental monitoring and material science. Proper use of a fluorescence spectrophotometer is critical to the accuracy and reproducibility of experimental results. The following are some things to pay attention to when using a fluorescence spectrophotometer:

1. Calibrate the instrument: Before performing a fluorescence analysis experiment, first calibrate the fluorescence spectrophotometer. Calibrating the instrument can ensure the accuracy of the measurement results and improve the reliability of the experiment.

2. Sample preparation: Proper sample preparation is the key to accurate experimental results. Before the fluorescence analysis experiment, the sample needs to be properly processed to ensure that the dilution and transparency of the sample meet the requirements of the instrument measurement.

3. Select the appropriate excitation wavelength and emission wavelength: According to different sample characteristics, select the appropriate excitation wavelength and emission wavelength for measurement. The correct wavelength selection can maximize the sensitivity and accuracy of fluorescence analysis.

4. Control the experimental environment: When conducting fluorescence analysis experiments, pay attention to controlling the stability of the experimental environment to avoid the interference of external light sources and vibrations on the experimental results.

5. Data analysis and recording: After completing the fluorescence analysis experiment, the data should be accurately analyzed and recorded, including the measurement of fluorescence intensity, sample processing methods and experimental conditions, for subsequent data processing and result verification.

By following the above precautions, we can use the fluorescence spectrophotometer for fluorescence analysis experiments more accurately and obtain reliable experimental results. I hope this article can help you better master the fluorescence analysis technology and improve the efficiency and accuracy of the experiment.

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