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[Chemical Knowledge]：QPCR one-step sample addition attention, the experiment is worry-free from the details

Read： 899 Time：6months ago Source：Transform the World with Simplicity

In qPCR one-step experiment, adding sample is a very important step, and good adding sample can greatly improve the accuracy and success rate of the experiment. Below, we will share some notes on qPCR one-step sampling, hoping to help the experimenter.

should pay attention to the preparation of samples. Be sure to prepare the sample well before dosing, including steps such as extraction, purification, and concentration testing. Ensure that the quality and concentration of the sample meet the experimental requirements, so as to ensure the accuracy of the experimental results.

should pay attention to the handling of samples. When adding samples, avoid bubbles and overflow, especially when adding samples in microtubes. It is recommended to use a special sample gun and micro tube to avoid possible errors during manual sample addition.

should pay attention to the selection and addition of reagents. Select the appropriate reagent according to the needs of the experiment and add it to the sample accurately according to the requirements in the operation manual. In the process of adding reagents, attention should be paid to prevent cross-contamination and mixing to avoid affecting the experimental results.

should pay attention to the labeling. After the completion of the sample, the sample should be labeled in a timely manner, including the test tube number, experimental date, reagents used and concentration and other information, in order to facilitate subsequent data analysis and results comparison. Through the above precautions,

hope to help everyone to be more meticulous and thoughtful in qPCR one-step experiment, so as to improve the success rate and accuracy of the experiment. Experiment without worry, starting from the details.

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