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[Chemical Knowledge]：How to use low melting point agarose gel correctly?

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Low melting point agarose gel is commonly used in biological laboratory, which is widely used in nucleic acid electrophoresis, protein electrophoresis and other experiments. It has the advantages of convenient use, fast gel speed and high resolution, but some matters need to be paid attention to during use to ensure the accuracy and safety of the experimental results. The following are precautions for the use of low melting point agarose gel:

1. Pay attention to the temperature of the gel: When preparing the gel, choose the appropriate temperature according to the experimental requirements. Under normal circumstances, a temperature of about 60°C is a common use temperature, but for some special experiments, the temperature may need to be adjusted. It is recommended to read the instructions carefully or refer to relevant literature before use.

2. Pay attention to the preparation concentration: the preparation concentration of low melting point agarose gel will affect the gel speed and resolution, so the agarose powder should be accurately weighed during the preparation process, and the operation should be carried out according to the preparation method in the instructions. After the preparation is completed, the quality of the gel should be checked in time to ensure that there is no abnormality.

3. avoid cross contamination: in the process of using agarose gel, to avoid the problem of cross contamination. In the process of preparing the gel, loading the sample and staining, pay attention to the use of clean tools and reagents to avoid contamination between different samples.

4. Safe use of agarose gel: agarose powder has a certain degree of irritation. Avoid inhalation and contact with eyes and skin when using it. Wear protective glasses and gloves during operation, and clean it in time after use.

should also properly dispose of the waste of agarose gel to avoid environmental pollution.

the correct use of low-melting-point agarose gel is very important for the accuracy of experimental results. I hope that the precautions introduced in this article can help you better use and manage agarose gel and provide guarantee for experimental work.

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