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[Chemical Knowledge]：How to correctly use ficoll cell separation solution?

Read： 473 Time：7months ago Source：Transform the World with Simplicity

In cytological research, cell separation and purification is a very important part of the work. As a common cell separation reagent, ficoll cell separation solution has the advantages of good separation effect and simple operation, and is widely used in cytology experiments. However, if used improperly, it may affect the separation effect and even cause damage to the cells. Therefore, when using ficoll cell separation solution, there are some precautions to be followed.

should pay attention to the dilution ratio. Generally speaking, the dilution ratio of ficoll cell separation solution is 1:2, that is, 1 part of separation solution and 2 parts of cell suspension are mixed evenly. This ratio is chosen to ensure that the cells are not subjected to excessive pressure during the separation process, thereby maintaining the integrity of the cells.

should pay attention to the use of centrifuge tube. After the mixture is added to the centrifuge tube, it is necessary to pay attention to the cover of the tube cover to avoid the centrifugal force generated during the centrifugation process affecting the stability of the mixture.

in the process of contact with the centrifuge tube, but also as gently as possible, to avoid violent oscillation or collision centrifuge tube, so as not to cause unnecessary interference to the mixture.

In addition, temperature control is critical. When using ficoll cell separation solution, it is important to keep the ambient temperature stable and avoid exposure to direct sunlight. Temperature fluctuations may affect the cell separation process, so the test should be carried out in a constant temperature incubator or other stable temperature environment.

pay attention to hierarchical extraction. When centrifugation is complete, multiple layers of liquid are formed in the centrifuge tube, as cells of different densities are separated by the ficoll separator. At this time, use a pipette or tip to remove the required cell suspension from different levels to avoid mixing cells of different densities.

In summary, the correct use of ficoll cell separation solution is essential. Only by strictly following the precautions for use can the effect of cell separation be guaranteed and the integrity and purity of the cells be ensured. I hope that the above content can help everyone, so that everyone can achieve better experimental results in cytological research.

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