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[Chemical Knowledge]：Matters needing attention in orange G solution

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Orange G is a commonly used biochemical reagent, widely used in cell and tissue staining. In the process of mixing orange G, we need to pay attention to the following aspects:

1. Laboratory safety
Orange G is a chemical reagent, and attention should be paid to laboratory safety when using it. In the process of liquid preparation, wearing laboratory safety glasses and gloves is essential. At the same time, do not place other experimental items on the dispensing table to avoid contamination of orange G.

2. The ratio of
orange G is usually 1:1000, that is, 1mg of orange G is dissolved in 1ml of solvent. When mixing liquid, accurate weighing and calculation are required to ensure the accuracy of the mixing ratio.

3. solvent selection
commonly used orange G solvents are ethanol, acetone, choose the right solvent for orange G dissolution and dyeing effect has a direct impact. When selecting the solvent, it is necessary to consider the solubility of orange G and its effect on cells, and select the appropriate solvent for solution preparation.

4. Liquid preparation operation
In the process of liquid preparation of orange G, attention should be paid to avoid oxidation and light. After the preparation is completed, it should be sealed in a dark bottle as soon as possible and stored in a cool and dry place. The preparation of

orange G requires gentle stirring to avoid bubbles and affect the subsequent dyeing effect.

5. Cleaning and waste
After the orange G solution is completed, it is necessary to clean the used containers and appliances in time, and properly dispose of waste liquid and waste items. Avoid orange G residues or environmental pollution.

above are some matters needing attention when mixing orange G solution, hoping to be helpful to your experimental work. I wish you good results in the experiment!