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[Chemical Knowledge]：How to properly use PBS buffer to clean tissues? Master these key points to ensure safe and effective operation!

Read： 847 Time：7months ago Source：Transform the World with Simplicity

The use of PBS buffer to clean tissue is a common procedure in biomedical research, the correct cleaning process can effectively retain the structure and function of the organization, but if the improper operation will affect the experimental results. Let us understand some of the key points to note when using PBS buffer to wash tissue.

to ensure the quality of PBS buffer. When using PBS buffer in the laboratory, be sure to choose a brand product with reliable quality to ensure the accuracy of its composition and concentration. In addition, it is necessary to strictly follow the storage and use methods in the instructions to avoid the performance degradation due to improper storage or use of PBS buffer.

requires attention to the pH of the PBS buffer. When performing tissue washing, it is important to ensure that the pH of the PBS buffer is within an appropriate range, generally between 7.2 and 7.4. If the pH value deviates from this range, it will affect the stability and structure of the protein, which will affect the experimental results.

should pay attention to the time and temperature of tissue cleaning. In general, the cleaning time should not be too long, usually between 5 and 10 minutes is appropriate; at the same time, to be cleaned at a suitable temperature, generally at room temperature. Long-term cleaning or too high or too low temperature will affect the structure and function of the organization.

In addition, avoid air bubbles during cleaning. The generation of bubbles will affect the cleaning effect on the tissue, so try to avoid bubbles during operation. If bubbles do appear, they should be removed in time. After the

has cleaned the tissue, it is rinsed with sufficient deionized water. This step can effectively remove the PBS buffer remaining in the tissue, thereby avoiding the impact on the experimental results. During the flushing process, attention should also be paid to avoid bubbles to ensure the adequacy and uniformity of the flushing.

the correct use of PBS buffer to clean the tissue is an important step to ensure the accuracy of the experimental results. Only by mastering the correct operation points can the operation be safe and effective. It is hoped that through the introduction of this paper, we can better master the skills of using PBS buffer to clean the tissue, and achieve better results in the experiment.

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