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[Chemical Knowledge]：Operational considerations for qPCR detection technology

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qPCR(quantitative polymerase chain reaction) is a molecular biology technique used to determine the amount of a specific sequence in a sample of DNA, RNA, or protein. When performing qPCR testing, there are some operational considerations that require special attention to ensure the accuracy and reliability of the experiment.
Before
experimental operation, it is necessary to disinfect and prepare experimental instruments in strict accordance with laboratory specifications. Keeping the experimental operating environment clean and sterile is the first step to ensure the accuracy of the experiment.

During sample extraction and preparation, care should be taken to avoid contamination. Use aseptic techniques and avoid repeated freezing and thawing of samples to avoid RNA degradation and bias in experimental results. When preparing and diluting experimental samples, we must strictly follow the requirements of the experimental scheme to avoid errors in experimental data due to operational errors. When preparing qPCR reagents,

need to use accurate measuring cylinders and pipettes, and avoid bubbles and droplet residues during the whole preparation process. When performing the PCR amplification reaction, care should be taken to avoid residual air in the amplification reaction tube and to avoid excessive shaking of the amplification reaction mixture, which may lead to deviations in the PCR reaction results. When performing qPCR detection, it is also necessary to ensure the stability and accuracy of the experimental instruments, and to verify and maintain the experimental instruments in time to ensure the accuracy of the experimental results. When

analyze the experimental data and interpret the results, it is necessary to combine the experimental design and strictly follow the standard operating procedure to avoid the error of the experimental results due to the error of the data analysis method.

qPCR detection is a widely used molecular biology technology, but some details need to be paid special attention to in order to ensure the accuracy and reliability of the experimental results.

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