Phenol ketone products

Plastic rubber

Current Location： Home > News > Chemical Knowledge > Precautions for proper use of glycogen PAS staining

[Chemical Knowledge]：Precautions for proper use of glycogen PAS staining

Read： 737 Time：8months ago Source：Transform the World with Simplicity

Glycogen PAS staining is a commonly used histological staining method to detect the content and distribution of glycogen in tissues. The correct use of the method can improve the staining effect and help researchers to accurately observe and analyze the glycogen of tissue samples. The following are matters needing attention when using glycogen PAS staining:

1. Specimen fixation: Before glycogen PAS staining, tissue specimens need to be appropriately fixed. The tissue specimen is usually fixed with a fixative such as formalin, and the fixation time and the concentration of the fixative need to be adjusted according to the specific experimental requirements.

2. Dewaxing and washing with water: Before staining, the wax in the specimen needs to be thoroughly removed and washed with water. The wax residue will affect the dyeing effect, and the incomplete washing will also lead to the decline of dyeing quality.

3. Alkaline water digestion: Before using PAS staining, tissue specimens need to be treated with alkaline water digestion. This step can make the glycogen color more in the dyeing process and improve the dyeing effect.

4. dyeing time control: in the PAS dyeing, need to control the dyeing time. Too long dyeing time will lead to excessive dyeing, affecting the observation and analysis; dyeing time is too short will lead to insufficient dyeing, can not accurately reflect the distribution of glycogen in the tissue. Therefore, it is necessary to strictly control the dyeing time during the dyeing process.

5. Dyeing quality control: After dyeing, the dyeing results need to be controlled. The staining of tissue specimens can be observed by microscope, and the staining effect can be evaluated according to the experimental requirements.

Precautions for proper use of glycogen PAS staining are essential for obtaining clear staining results and accurate experimental results. Only in strict compliance with the operating procedures, can we ensure the stability and comparability of the staining effect, so as to provide reliable data support for subsequent histological studies.

Previous： Matters needing attention and steps of cadmium chloride test

Next： Safety precautions for the transportation of sulfuric acid

News Categories