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[Chemical Knowledge]：Correct Use Method and Precautions of n-Butanol Stratification

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n-Butanol is an important organic compound, which is often used as a solvent in the laboratory. In the laboratory, n-butanol is often used for layered extraction, but some matters need to be paid attention to during use to ensure the smooth progress of the experiment and personal safety. This article will introduce the correct use of n-butanol stratification and precautions.

to use the correct laboratory equipment
when performing n-butanol stratification experiments, you need to use appropriate laboratory equipment. First of all, it is necessary to ensure that the centrifuge used can meet the requirements of the experiment, and its speed and centrifugal force need to meet the requirements of n-butanol stratification.

also need to use reliable centrifuge tubes and test tubes for layering to avoid accidents in the experiment. It is

to master the correct layering operation method
When carrying out the n-butanol layering experiment, it is necessary to master the correct layering operation method. First of all, it is necessary to ensure that the mixture to be separated is sufficiently cooled to prevent the solvent from volatilizing too fast to affect the stratification effect.

the stratification operation, it is necessary to gently add n-butanol, and pay attention to avoid the generation of bubbles, so as not to affect the effect of stratification. In the process of centrifugation, the appropriate centrifugation time and speed should be selected to ensure that the two phases are separated clearly.

Pouring and Storing n-Butanol Correctly
After the n-butanol stratification experiment is completed, n-butanol needs to be poured and stored correctly. First of all, make sure that no layer of the mixture is poured out during the pouring process, so as to avoid the re-mixing of the mixture and the failure of the experiment. When storing n-butanol in

, it is necessary to select a suitable container and store it in a cool and ventilated place to avoid heat and direct sunlight and volatilization.

Avoid contact and inhalation of n-butanol
When conducting n-butanol stratification experiments, it is necessary to strictly abide by laboratory safety regulations and avoid contact and inhalation of n-butanol. When conducting experiments, you should wear appropriate laboratory protective equipment, such as gloves, goggles, etc., to ensure personal safety.
After
experiment, wash hands and change clothes in time to avoid harm to the body caused by n-butanol residue.

In summary, n-butanol is a commonly used organic solvent. There are some matters that need to be paid attention to during the layering process, including using the correct laboratory equipment, mastering the correct layering operation method, pouring and storing n-butanol correctly, and Avoid exposure and inhalation of n-butanol. Only by strictly abiding by the laboratory safety standards and mastering the correct hierarchical operation method can we ensure the smooth progress of n-butanol stratification experiment and the safety of laboratory personnel. It is hoped that this paper can help experimenters to better use n-butanol for layering operations.