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[Chemical Knowledge]：Explore the principle of inverted fluorescence microscope, pry the mystery of life

Read： 265 Time：20months ago Source：Transform the World with Simplicity

As a high-tech microscope, inverted fluorescence microscope is widely used in various fields. Although beginners may not understand its specific principles, but with the deepening of understanding, you will find that the principle of the microscope is relatively simple and efficient.

So, what exactly is an inverted fluorescence microscope? An inverted fluorescence microscope is a high-resolution microscope that changes the relative position of the sample and the objective lens so that the sample is placed at the bottom of the microscope. This particular design is ideal for observing cell cultures, tissue engineering and biomaterials for biological experiments on culture dishes or plates.

is different from the ordinary optical microscope, inverted fluorescence microscope is irradiated from the bottom of the sample, and then through the objective lens magnification, through the open mirror back to form the inverted fluorescence microscope illumination of the great penetration.

Under the excitation of the fluorescent probe, the substance can emit more intense and brighter fluorescence. This allows the inverted fluorescence microscope to observe the fluorescence of calcium, ammonia, acid and DNA and many other biological molecules, and even observe the composition of nano-scale biological molecules and their molecular pairing.

This inverted fluorescence observation method plays a very important role in life science research and patient diagnosis and treatment. For example, in the medical field, the use of an inverted fluorescence microscope allows for rapid diagnosis and process tracking of skin pathological sections, allowing for smooth treatment. In the field of biological science, inverted fluorescence microscope can be used to observe cells, cell tissue, metabolism and pathological changes.

In addition, inverted fluorescence microscopy is also widely used in three-dimensional microscopic imaging, long-term tracking, pharmacology and cell biology research. With the help of inverted fluorescence microscope, we can study the essence of life and the living environment of cells in depth, which provides a new way of thinking for various biological science research, medical diagnosis and treatment.

In a word, although the principle of inverted fluorescence microscope is slightly complicated compared with ordinary microscope, this microscope can also break through the technical difficulties of many life science research. With the continuous development of science and technology, I believe that inverted fluorescence microscope will be able to bring more scientific and technological achievements for people.

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