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[Chemical Knowledge]：The role of phosphate buffer and its application in biology

Read： 1821 Time：22months ago Source：Transform the World with Simplicity

Phosphate buffer is a common buffer in the laboratory, its components mainly include phosphate monobasic salt, phosphate binary salt, its preparation method is relatively simple. The role of phosphate buffer in the experiment is mainly to stabilize the pH value of the sample and prevent the error of the experimental results caused by the change of pH value. The principle of action of

phosphate buffer is to utilize the two proton chemical bonds of the phosphate molecule. In the buffer, when the pH gradually increases, the phosphate molecule will first lose a proton, so that the pH can be maintained in the lower range. As the pH gradually decreases, the phosphate molecule accepts a proton, allowing the pH to be maintained in the higher range. Since the phosphate molecule can accept and release protons over a wide range of pH values, the phosphate buffer can maintain a stable pH value under a variety of pH conditions.

phosphate buffer is widely used in biology. For example, it is often used in cell culture, enzyme catalytic reaction and other experiments. Cells can only grow and divide within a specific pH range, and phosphate buffer can provide a good pH range to promote cell growth and division. In the enzyme-catalyzed reaction, the enzyme activity is greatly affected by the pH value. When the pH value exceeds a certain range, the activity of the enzyme will be reduced or completely inactivated. Selection of a suitable phosphate buffer can help maintain the activity of the enzyme.

phosphate buffer plays a vital role in the laboratory, which can stabilize the pH value, reduce experimental errors, and ensure the accuracy of experimental data. Understanding the principle of its action and the scope of its application, and selecting the appropriate phosphate buffer will contribute to the success of the experimental results.

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